ABSTRACT
Objective Rho signaling component α-catulin,is a cytoskeletal linker protein and plays an important role in apoptotic and senescence resistance,cytoskeletal reorganization,mobility,invasion and epithelial to mesenchymal transition(EMt)of cancer cells. Methods Here,we ex-amined α-catulin expression in squamous epithelium,dysplasia and cancer of head and neck on tissue microarrays by immunostaining. Its expres-sion was compared with clinicopathological parameters and survival rate of cancers. Results It was found that α-catulin expression level was signifi-cantly higher in primary cancers than that in normal squamous epithelium and dysplasia(P 0.05). Cox′s proportional hazard model indicated that distant metastasis and tNM staging were independent prognostic factors for overall survival of the patients with head and neck squamous carcinoma(HNSCC,P < 0.05). Conclusion these findings suggested that up-regulated expression of α-catulin protein may play an important role in the pathogenesis of HNSCC,which might be employed as a potential marker for tumorgenesis of HNSCCs.
ABSTRACT
Objective To construct and confirm the JC virus(JCV) T antigen expression plasmid using mouse keratin 19 (K19) promoter specific for the gastric epithelial cells.Methods The Ndel site was mutated by FCR with Bell insertion at both sides.The DNA fragment digested by Bcl Ⅰ was ligated with the plasmid containing K19 promoter via Bam Ⅰ site.The DNA sequence was confirmed by restriction enzyme digestion and direct DNA sequencing.Cytokeratin 19 protein was examined to screen gastric carcinoma cell for transfection of K19-JCV T antigen expression plasmid by immunohistochemistry.The Western blot was employed to detect the JCV T antigen expression in the gastric carcinoma transfectant.Results K19-JCV T antigen expressing plasmid was successfully constructed.The ACS strongly expressed cytokeratin 19 protein and was selected for the transfection of K19-JCV T antigen expressing plasmid.JCV T antigen was positively expressed in the AGS transfectant.Conclusion The synonymous mutation and compatible ligation are useful in the plasmid construction.The methy lation of restriction enzyme should be considered.It is meaning for the transgenic animal model of gastric carcinoma to successfully construct the JC virus T antigen expression plasmid in gastric mucosa.